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Nichirei Corporation fast red ii substrate kit
Fast Red Ii Substrate Kit, supplied by Nichirei Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fast+red+substrate+kit/fast+red+ii+substrate+kit/pm38923622-56-19-25
Average 90 stars, based on 1 article reviews
fast red ii substrate kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Staining:

Article Title: Generation of cleidocranial dysplasia-specific human induced pluripotent stem cells in completely serum-, feeder-, and integration-free culture
Article Snippet: Fluorescence images were taken using a Zeiss inverted LSM 700 confocal microscope (Carl Zeiss, GmbH, Germany). .. Alkaline phosphate (ALP) staining was performed using a Fast Red substrate kit (Nichirei) according to manufacturer’s instruction. .. Total RNA was extracted from iPSCs using the Illustra RNA spin Mini Isolation kit (GE Healthcare UK Ltd, Buckinghamshire, England) according to manufacturer’s instruction. cDNA was synthesized from 1 μg of total RNA using High capacity RNA-to cDNA master mix (Applied Biosystems, Carlsbad, CA) and RT-PCR reactions were performed with KOD-FX neo (Toyobo, Japan) using primers described in Supplementary Table .

Article Title: Alpha-internexin and altered CIC expression as a supportive diagnostic marker for oligodendroglial tumors with the 1p/19q co-deletion.
Article Snippet: a-Internexin (INA) has been proposed as a biomarker of oligodendroglial tumors with the 1p/19q codeletion.. On the other hand, sequence studies have recently linked the CIC mutation and subsequent altered CIC expression to the 1p/19q co-deletion in oligodendroglial tumors.. We assessed the usability of combination immunohistochemical analysis using CIC and INA as a surrogate tool for the 1p/19q status in 39 cases of oligodendroglial tumors.

Activity Assay:

Article Title: Integrins regulate mouse embryonic stem cell self-renewal.
Article Snippet: Cells were counted everyday with a Coulter particle counter (Beckman Coulter, Hialeah, FL, http:// www.beckmancoulter.com). .. The alkaline phosphatase (AP) activity of mES cells cultured in the defined medium for 4 days on adhesion molecule-coated six-well plates in ESF7 or on a gelatin-coated plate in CEM was detected with a Fast Red substrate kit (Nichirei, Tokyo, http://www.nichirei. co.jp/english/index.html) as described previously [27, 32]. ..

Cell Culture:

Article Title: Integrins regulate mouse embryonic stem cell self-renewal.
Article Snippet: Cells were counted everyday with a Coulter particle counter (Beckman Coulter, Hialeah, FL, http:// www.beckmancoulter.com). .. The alkaline phosphatase (AP) activity of mES cells cultured in the defined medium for 4 days on adhesion molecule-coated six-well plates in ESF7 or on a gelatin-coated plate in CEM was detected with a Fast Red substrate kit (Nichirei, Tokyo, http://www.nichirei. co.jp/english/index.html) as described previously [27, 32]. ..

Incubation:

Article Title: Monocyte Chemoattractant Protein-1 Is an Essential Inflammatory Mediator in Angiotensin II-Induced Progression of Established Atherosclerosis in Hypercholesterolemic Mice
Article Snippet: Air-dried cryostat sections were fixed in acetone and stained with the respective antibody: anti-mouse monocyte/macrophage monoclonal antibodies (MOMA-2; Serotec), anti-alpha-SM actin monoclonal antibodies (alkaline phosphatase conjugated; Sigma Chemical), anti-human MCP-1, CCR2, MMP-13 polyclonal antibodies (Santa Cruz Biotechnology, Santa Cruz, CA), anti-mouse MMP-9 polyclonal antibodies (Santa Cruz Biotechnology), and anti-rabbit tissue factor monoclonal antibody (American Diagnostica), as described previously.18 Respective nonimmune IgGs (Dako) were used as negative controls. .. After incubation with the appropriate biotinylated affinity-purified secondary antibodies (Nichirei, Tokyo), the sections were incubated with alkaline phosphatase-labeled streptavidin solution (Nichirei) and visualized using a fast red substrate kit (Nichirei). ..

Affinity Purification:

Article Title: Monocyte Chemoattractant Protein-1 Is an Essential Inflammatory Mediator in Angiotensin II-Induced Progression of Established Atherosclerosis in Hypercholesterolemic Mice
Article Snippet: Air-dried cryostat sections were fixed in acetone and stained with the respective antibody: anti-mouse monocyte/macrophage monoclonal antibodies (MOMA-2; Serotec), anti-alpha-SM actin monoclonal antibodies (alkaline phosphatase conjugated; Sigma Chemical), anti-human MCP-1, CCR2, MMP-13 polyclonal antibodies (Santa Cruz Biotechnology, Santa Cruz, CA), anti-mouse MMP-9 polyclonal antibodies (Santa Cruz Biotechnology), and anti-rabbit tissue factor monoclonal antibody (American Diagnostica), as described previously.18 Respective nonimmune IgGs (Dako) were used as negative controls. .. After incubation with the appropriate biotinylated affinity-purified secondary antibodies (Nichirei, Tokyo), the sections were incubated with alkaline phosphatase-labeled streptavidin solution (Nichirei) and visualized using a fast red substrate kit (Nichirei). ..



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